我国东、西方蜜蜂中七种病毒感染状况的调查

doi:10.11843/j.issn.0366-6964.2015.10.015

Abstract

Abstract:

This study aimed to investigate the epidemiology of bee viruses in Apis cerana and Apis mellifera and analyze the influence of different locations and beekeeping environment on virus infection.During 2012-2013，50 colonies of Apis cerana and 108 colonies of Apis mellifera were collected from 8 provinces and municipalities.RT-PCR was used to detect seven viruses，deformed wing virus(DWV)，black queen cell virus(BQCV)，sac brood virus(SBV)，Kashmir bee virus(KBV)，chronic bee paralysis virus(CBPV)，acute bee paralysis virus(ABPV) and Israeli acute paralysis virus(IAPV).The results showed that no KBV was detected in any samples.BQCV was most prevalent among the six detected viruses，and DWV was the second in both honey bee species.Infection rate of SBV in Apis mellifera is significantly higher than in Apis cerana(P<0.01).CBPV，ABPV and IAPV were present at low levels in both species.Among the four provinces for Apis cerana samples，IAPV was found in Liaoning and Hainan，CBPV was present in Fujian and Liaoning while ABPV was only detected in Guangdong.DWV，BQCV and SBV distributed in all seven provinces where Apis mellifera were collected.But SBV infected at lower level in Fujian than the other locations(P<0.01).Five viruses were present in Xinjiang，Beijing and Liaoning，and four viruses were detected in Shanxi，Shandong，Hainan and Fujian.For the three types of beekeeping areas，six viruses were found in Apis mellifera isolated areas，while five viruses in mixed Apis mellifera and Apis cerana areas and only three viruses in Apis cerana isolated areas.Simultaneous multiple viruses infection was common in both species，with most samples were infected with DWV and BQCV or DWV，BQCV and SBV.These results indicated that BQCV and DWV were the most prevalent viruses in both species.The distribution and infection rate of the six detected viruses varied in different locations and beekeeping areas，but multiple-viruses infection was common in two honeybee species.

CLC Number:

S895.13

DING Gui-ling，SHI Wei. Investigation of Seven Viruses in Apis cerana and Apis mellifera of China[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(10): 1822-1828.

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Investigation of Seven Viruses in Apis cerana and Apis mellifera of China

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